A question list of Diphenylamine for you

1.Diphenylamine test for deoxyribose?
Answer1:
When adding diphenylamine to deoxyribose you will get a dark deep purple color. If you react diphenylamine with crude DNA you will get a pink-violet color. DIphenylamine test is quantitative and the darker the color the greater the concentration of DNA in the solution

2.What is diphenylamine test for deoxyribose for?
Answer2:
It can be used to test for the presence of DNA in a substance. The test consists of diphenylamine(CAS NO.: 122-39-4) in acid, which reacts with the deoxyribose in the DNA to produce a blue color. The intensity of the blue color is in direct proportion to the concentration of DNA.

One major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. DNA can be identified chemically with the Dische diphenylamine test. The reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex

3.What is responsible for the formation of the absorbed color of DNA with diphenylamine?
Answer 3:
When you heat DNA in acid the 2-deoxyribose is converted to w-hydroxylevulinyl aldehyde, which reacts with the compound, diphenylamine, to produce a blue-colored compound.

4.Why does blue colour appears in diphenylamine test?
Answer 4:
Blue color appears in diphenylamine test because of the reaction of nitrates.

5.Detection of DNA with Diphenylamine?
Answer 5:
A diphenylamine indicator is often used for the detection of DNA. Diphenylamine is an organic compound and classified as an aromatic amine due to the presence of a benzene ring in its chemical makeup. Chemical hydrolysis of DNA must take place when looking for it using diphenylamine. The DNA concentration in an organism is measured by looking for the absorbency rate of the diphenylamine. DNA molecules are the building blocks of life and contain specific genetic coding for the organism it is contained in.

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